The most common mode of public transport in Prague is undoubtedly the metro.  Although, over time the number of accessible stations have gradually increased, yet transport using the metro requires overcoming a few obstacles.

The two most serious being:  the gap between the platform and the carriage, and the difference in height.  This becomes especially complicated for those using electric wheelchairs.  The reason for this is due to different types of trains, which run in the Prague metro; and load intensity of the carriages. For this reason, special rubber "rectifying combs" and ramps are placed on the platform edge.

You can find more information about accessibility of metro stations here: http://www.dpp.cz/en/barrier-free-travel/metro/